We apply several techniques and methodologies in the lab that are rooted in immunochemistry, biochemistry, microbiology, molecular biology, immunology, and virology. The main lab includes a thermocycler, warm incubators and shakers, temperature-controlled table-top centrifuges and microcentrifuges, several heat blocks, a +4°C lab fridge, -20°C freezers, a -80°C freezer, protein and nucleic acid gel-electrophoresis apparatuses, a Nanovue, an ELISA plate reader and plate washer, a high-performance microplate luminometer, an ÄKTA FPLC with MALS and RI detectors and an Octet RED96e biolayer interferometer. Multiple desktop PCs are available for data analyses and other research-related work.
Directly connected to the main lab are a dedicated tissue culture room, a chemicals room and and a large shared walk-in cold room. The tissue culture room includes BSCs, a temperature-controlled table-top centrifuge, a large-capacity CO2 incubator, two non-CO2 incubators, an additional thermocycler, an inverted microscope and a -20°C freezer. The chemicals room is used for buffer and reagent preparation and chemicals storage and the cold room for storage of items by lab members and for conducting overnight experiments that require refrigeration.
Lab members also have nearby access to shared equipment, including a GelDoc system, a state-of-the art qPCR instrument, a 10X Chromium Controller, and a Flow Cytometry Laboratory with instruments for cell analyses and sorting. Autoclaves and a dishwashing system are also available nearby for cleaning and sterilizing labware and for disposal of biohazardous waste. Animal work is supported by Animal Care Services and contract research organizations.